Uncoated Human F3/TF (Tissue Factor) ELISA Kit (E-UNEL-H0373)

Name: Uncoated Human F3/TF (Tissue Factor) ELISA Kit
Brand: Elabscience
SKU: E-UNEL-H0373
Price: 399.0 USD
Availability: InStock

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Uncoated Human F3/TF (Tissue Factor) ELISA Kit - 1

All	Size	Price	Qty
	96T*5	$ 399.00	- +
	96T*15	$ 958.00	- +

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Product Summary

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	24 h
Reactivity	Human
Specificity	This kit recognizes Human F3/TF in samples.No significant cross-reactivity or interference between Human F3/TF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T5 / 96T15
Storage	-20℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/ml	OD1	OD2	Mean OD	Corrected OD
500	2.266	2.314	2.290	2.203
250	1.557	1.569	1.563	1.476
125	0.995	1.017	1.006	0.919
62.5	0.556	0.580	0.568	0.481
31.25	0.324	0.322	0.323	0.236
15.63	0.190	0.200	0.195	0.108
7.81	0.146	0.134	0.140	0.053
0	0.089	0.085	0.087	-

Background

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.